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PBS缓冲液和Hanks缓冲液的区别 - 实验方法 - 丁香通
PBS缓冲液和Hanks缓冲液的区别 - 实验方法 - 丁香通
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首页 实验方法 遗传学实验技术 经典遗传学实验 PBS缓冲液和Hanks缓冲液的区别
PBS缓冲液和Hanks缓冲液的区别
PBS缓冲液和Hanks缓冲液的区别
关键词： pbs 缓冲液
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万能缓冲液PBS
我实验时经常用到PBS缓冲液，但是很多文献里面也用到了Hanks缓冲液，不知道这两种缓冲液在细胞培养 方面有什么区别吗?也就是说它分别适合于哪些情况?
PBS ：磷酸缓冲盐溶液——具有pH缓冲作用的等渗盐溶液。PBS溶液又称磷酸盐缓冲溶液，一般选择K2HPO4和KH2PO4配制，因为钠盐溶解的较慢。根据不同pH的溶液，称量不同质量的磷酸盐，也可以用pH计调溶液的pH。PBS一般用作支持电解质。
其配置方法如下：采用去离子水、KH2PO4和Na2HPO4·2H2O配制PBS溶液，按以下步骤进行：
(1) 配制1/15mol/L KH2PO4，即每升水中溶解9.078克KH2PO4;
(2) 配制1/15mol/LNa2HPO4·2H2O，即每升水中溶解11.876克Na2HPO4·2H2O;(3) 将18.2%(体积分数)KH2PO4溶液和81.8%Na2HPO4·2H2O混合;最终 测定PBS液的PH值约为7.4。其作用接近于生理盐水，但是在实验室内比生理盐水的用途要广泛。
Hanks：平衡盐溶液——无机盐和葡萄糖浓度接近大部分动植物 细胞的水平，可作为动植物细胞短期培养(保存)。 Hank's配方:NaCl 8.01;KCl 0.4;CaCl2 0.14; NaHCO3 0.35;KH2PO 4 0.06;Glucose 0.34(g/l); D-Hank' s:不含钙离子、镁离子、葡萄糖。( T7 e# L7 e, K2 N
相比较而言，hanks液中盐成分较PBS 复杂，且有葡萄糖，可为细胞提供简单的营养，而pbs中只有磷酸根，钠，钾，氯离子，只是最简单的盐平衡缓冲液，不能为细胞提供暂时的营养 。
关键要看你用这些液体来洗细胞还是存储细胞了
PBS 、hanks各都有两种，一种是含钙镁的，另一种是不含钙镁的：DPBS,DHANKS。 r) u7 n6 e3 R% P
hanks比PBS成分稍微复杂点，一般细胞培养时用DPBS就够，Dhanks一般用到原代细胞消化分离操作实验中,一般培养用PBS就够了，感觉DHANKS上场的实验比较高级点哎，对于保护细胞活力等很有好处。
HANKS对细胞略好， 不过一般洗涤等用PBS足够了。 分离时，两者皆可， 多数推荐HANKS，实际上差不多。如果分离时消化时间较长， 建议HANKS
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Hank's液_百度百科
's液_百度百科 网页新闻贴吧知道网盘图片视频地图文库资讯采购百科百度首页登录注册进入词条全站搜索帮助首页秒懂百科特色百科知识专题加入百科百科团队权威合作下载百科APP个人中心收藏查看我的收藏0有用+10Hank's液播报讨论上传视频平衡盐溶液Hank's液，主要用于细胞培养取材时组织块的漂洗、细胞的漂洗、配制其他试剂等。中文名Hank's液属 性平衡盐溶液用 途细胞培养取材时组织块的漂洗简 称H目录1简介2配制简介播报编辑D-Hank's与Hank's的一个主要区别在于前者不含有钙和镁离子，因此D-Hank's常用于配制胰酶溶液。配制播报编辑Hanks 液是生物医学实验中最常用的无机盐溶液和平衡盐溶液（Balanced Salt Solution,BSS ），简称H。主要用于配制培养液，稀释剂和细胞清洗液，而不能单独作为细胞组织培养液。原液A---NaCl160g-MgSO4·7H2O2g-KCl8g-Mg Cl2·6H2O2g-CaCl22.8g-溶于1000ml双蒸水-原液 B--（1）Na2HPO4·12H2O3.04g-KH2PO41.2g-葡萄糖20.0g-溶于800ml双蒸水-（2）0.4%酚红溶液：取酚红0.4g，置玻璃研钵中，逐滴加入0.1N NaOH，并研磨，直至完全溶解，约加0.1N NaOH 10ml。将溶解的酚红吸入100ml量瓶中，用双蒸水洗下研钵中残留酚红液，并入量瓶中，最后补加双蒸水至100ml。--将（1）液和（2）液混合，补加双蒸水至1000ml，即为原液B。-应用液---原液A1份-原液B1份-双蒸水18份-混合后，分装于200ml小瓶中，10磅高压蒸气灭菌15分钟，临用前用无菌的5.6% NaHCO3调pH至7.2~7.6-新手上路成长任务编辑入门编辑规则本人编辑我有疑问内容质疑在线客服官方贴吧意见反馈投诉建议举报不良信息未通过词条申诉投诉侵权信息封禁查询与解封©2024 Baidu 使用百度前必读 | 百科协议 | 隐私政策 | 百度百科合作平台 | 京ICP证030173号 京公网安备110000020000Hank's液_百度百科
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Hank's液，主要用於細胞培養取材時組織塊的漂洗、細胞的漂洗、配製其他試劑等。
中文名
Hank's液
屬 性
平衡鹽溶液
用 途
細胞培養取材時組織塊的漂洗
簡 稱
H
目錄
1
簡介
2
配製
Hank's液簡介
D-Hank's與Hank's的一個主要區別在於前者不含有鈣和鎂離子，因此D-Hank's常用於配製胰酶溶液。
Hank's液配製
Hanks 液是生物醫學實驗中最常用的無機鹽溶液和平衡鹽溶液（Balanced Salt Solution,BSS ），簡稱H。主要用於配製培養液，稀釋劑和細胞清洗液，而不能單獨作為細胞組織培養液。原液A---NaCl160g-MgSO4·7H2O2g-KCl8g-Mg Cl2·6H2O2g-CaCl22.8g-溶於1000ml雙蒸水-原液 B--（1）Na2HPO4·12H2O3.04g-KH2PO41.2g-葡萄糖20.0g-溶於800ml雙蒸水-（2）0.4%酚紅溶液：取酚紅0.4g，置玻璃研缽中，逐滴加入0.1N NaOH，並研磨，直至完全溶解，約加0.1N NaOH 10ml。將溶解的酚紅吸入100ml量瓶中，用雙蒸水洗下研缽中殘留酚紅液，併入量瓶中，最後補加雙蒸水至100ml。--將（1）液和（2）液混合，補加雙蒸水至1000ml，即為原液B。-應用液---原液A1份-原液B1份-雙蒸水18份-混合後，分裝於200ml小瓶中，10磅高壓蒸氣滅菌15分鐘，臨用前用無菌的5.6% NaHCO3調pH至7.2~7.6-
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Hanks液的配制方法步骤及其注意事项 - 实验方法 - 丁香通
Hanks液的配制方法步骤及其注意事项 - 实验方法 - 丁香通
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首页 实验方法 细胞技术 细胞功能测定 Hanks液的配制方法步骤及其注意事项
Hanks液的配制方法步骤及其注意事项
Hanks液的配制方法步骤及其注意事项
关键词： 配制 方法 步骤
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Hanks 液是生物医学实验中极为常用的无机盐溶液和平衡盐溶液(Balanced Salt Solutions,简称 BSS)之一，简称HBSS。主要用途是用于配制培养液，稀释剂以及细胞清洗液，但是不能单独作为细胞/组织培养液。本详细表述了Hanks 液平衡盐溶液的配制方法和步骤。
原液A
&NBS p;
&NBS p;
&NBS p;
NaCl
160g
&NBS p;
MgSO4 ·7H2 O
2g
&NBS p;
KCl
8g
Mg Cl2 ·6H2 O
2g
CaCl2
2.8g
溶于1000ml双蒸水
原液 B
（1）
Na2 HPO4 ·12H2 O
3.04g
KH2 PO4
1.2g
葡萄糖
20.0g
溶于800ml双蒸水
（2）
0.4%酚红溶液：取酚红0.4g，置玻璃研钵中，逐滴加入0.1N NaOH，并研磨，直至完全溶解，约加0.1N NaOH 10ml。将溶解的酚红吸入100ml量瓶中，用双蒸水洗下研钵中残留酚红液，并入量瓶中，最后补加双蒸水至100ml。
将（1）液和（2）液混合，补加双蒸水至1000ml，即为原液B。
应用液
原液A
1份
原液B
1份
双蒸水
18份
混合后，分装于200ml小瓶中，10磅高压蒸气灭菌15分钟，临用前用无菌的5.6% NaHCO3 调pH至7.2~7.6
注意事项：
酚红对细胞有一定的毒性，可根据自己的实验选择一定的量，酚红所加的用量不一样，溶液的颜色也会有少许变化。此外配制hanks液所用的试剂必须达到一级GR或者二级AR规格。
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Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt Solution
Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt Solution
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HomeShop All ProductsCell Culture Media, Supplements, and ReagentsCell Culture Reagents, Antibiotics and SupplementsDissociation ReagentsCell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt SolutionGibco™Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt SolutionCatalog number: 13150016Related applications: Mammalian Cell CultureTechnical SupportCustomer ServiceGibco™Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt SolutionCatalog number: 13150016Related applications: Mammalian Cell CultureTechnical SupportCustomer ServiceCatalog Number13150016also known as 13150-016Unit Size100 mlPrice (USD)Contact Us ›Save to listCatalog NumberUnit SizePrice (USD)13150016also known as 13150-016100 mlContact Us ›Save to listProduct OverviewFiguresVideosRecommendationsRecommendationsDocumentsFAQCitations & ReferencesAdditional InformationRecommendationsGibco™ Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Hanks' Balanced Salt Solution (HBSS). Gibco™ Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco™ Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells. The dissociation protocol is available in our Technical Reference Library.For Research Use Only. Not for use in diagnostic procedures.SpecificationsChelatorsEDTACell LineHeLa, NIH 3T3ClassificationAnimal Origin-freeProduct TypeCell Culture Dissociation ReagentShelf Life24 MonthsSterilitySterile-filteredWith AdditivesEDTA, Earle's Balanced Salt Solution (EBSS)RemarksRoom Temperature StableShipping ConditionRoom TemperatureContents & StorageStorage conditions: 15–30°CShipping conditions: ambientShelf life: 24 months from date of manufactureFiguresDocuments & DownloadsCertificatesSearch by lot number or partial lot numberSearchRequest a CertificateFrequently asked questions (FAQs)Citations & ReferencesSearch citations by name, author, journal title or abstract textSearch
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Hanks' Balanced Salt Solution
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C0218
Hanks' Balanced Salt Solution
500ml
49.00元
碧云天生产的Hanks' Balanced Salt Solution，简称HBSS，含137.93mM NaCl, 5.33mM KCl, 4.17mM NaHCO3, 0.441mM KH2PO4, 0.338mM Na2HPO4, 5.56mM D-Glucose，pH值为7.1-7.5。
本Hanks' Balanced Salt Solution溶液经过过滤除菌，可以直接用于细胞培养过程中细胞的洗涤等常规用途。使用前不必再进行稀释或过滤除菌等任何处理。
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C0218
Hanks' Balanced Salt Solution
500ml
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1份
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4℃保存，半年有效。-20℃保存一年有效。本产品可以短期室温存放。
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在使用Hanks' Balanced Salt Solution的过程中要特别注意避免溶液被微生物污染。
本Hanks' Balanced Salt Solution溶液不含Ca2+与Mg2+，如需使用含有Ca2+与Mg2+的Hanks' Balanced Salt
Solution 溶液，请订购C0219 Hanks' Balanced Salt Solution(with Ca2+ & Mg2+)。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
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HBSS (10X), calcium, magnesium, no phenol red
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HomeShop All ProductsBuffers and StandardsLife Science BuffersHBSS (10X), calcium, magnesium, no phenol redGibco™HBSS (10X), calcium, magnesium, no phenol redCatalog number: 14065056Related applications: Mammalian Cell CultureTechnical SupportCustomer ServiceGibco™HBSS (10X), calcium, magnesium, no phenol redCatalog number: 14065056Related applications: Mammalian Cell CultureTechnical SupportCustomer ServiceCatalog Number14065056also known as 14065-056Unit Size500 mLPrice (USD)Contact Us ›Save to listCatalog NumberUnit SizePrice (USD)14065056also known as 14065-056500 mLContact Us ›Save to listCustomize this productProduct OverviewFiguresVideosRecommendationsRecommendationsDocumentsFAQCitations & ReferencesAdditional InformationRecommendationsHanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation. Concentrated forms of Gibco™ HBSS require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications. This HBSS is modified as follows:WithWithout• Calcium• Phenol Red• Magnesium• Sodium Bicarbonate• Glucose The complete formulation is available.For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.SpecificationsAdditiveNo Sodium PyruvateClassificationAnimal Origin-FreeDilution10 XProduct LineGibco™Product TypeHanks' Balanced Salt Solution (HBSS)Quantity500 mLSolution TypeHank's Balanced SaltFor Use With (Application)Variety of Cell Culture Applications; Such as Washing Cells Before Dissociation; Transporting Cells or Tissue Samples; Diluting Cells for Counting And Preparing ReagentsGreen FeaturesSustainable packagingOsmolality2700 - 3070 mOsm/kgpH4.4 - 4.7Phenol Red IndicatorNo Phenol RedShipping ConditionRoom TemperatureSterilitySterile-filteredFormLiquidManufacturing QualitycGMP-compliant under the ISO 13485 standardContents & StorageStorage conditions: 15°C to 30°CShipping conditions: Room temperatureShelf life: 24 months from date of manufactureFiguresDocuments & DownloadsCertificatesSearch by lot number or partial lot numberSearchRequest a CertificateFrequently asked questions (FAQs)Citations & ReferencesSearch citations by name, author, journal title or abstract textSearch
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Home›Life Sciences›Cell Culture & Transfection›Mammalian Cell Culture›Cell Culture Reagents›Balanced Salt SolutionsBalanced Salt SolutionsSee Navigation‹Cell Culture ReagentsBalanced Salt Solutions
›Cell Freezing Media
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How balanced salt solutions affect your cell culture
Balanced salt solutions maintain the structural and physiological integrity of cells in vitro. Gibco Balanced Salt Solutions are isotonic buffer systems that help keep mammalian cells within their physiological pH range and maintain high viability during short-term incubations.A balanced salt solution’s essential function is to maintain pH and osmotic balance as well as provide your cells with water and essential inorganic ions. Gibco Balanced Salt Solutions are available in many different formulations and formats.Please see below for the common formulation components found in balanced salt solutions and how they may impact your cell culture.Shop all balanced salt solutions Compare PBS & DPBS Compare HBSS & EBSS
View: balanced salt solution formulation guide
Choose the right balanced salt solution for your cell cultureComponentComponent rolePBSDPBSHBSSEBSSWith Ca2+ & Mg2+Cell adhesionNeeded when cells are to remain attached to culture substrate✓✓✓✓Without Ca2+ & Mg2+✓✓✓✓With glucose & pyruvateEnergy source and cell survival supportMay keep cells alive longer when culture medium is absent✓✓✓✓Without glucose & pyruvate ✓✓✓With phenol redpH indicatorExhibits gradual color change from yellow to pink when between pH 6.8 and 8.2 ✓✓Without phenol red ✓✓With sodium bicarbonateStabilizes pH in CO2 environmentsCan be used in CO2 rich environments such as cell culture incubators ✓✓Balanced salt solutions selection guidesAvailable in both liquid and powder form, all Gibco balanced salt solutions are manufactured in state-of-the-art cGMP, ISO-certified facilities, maintaining the highest quality and consistency for reproducible results. Regular testing includes osmolality, pH levels, stability, as well as testing against the presence of endotoxins, and bacterial and fungal contamination.For increased stability, all powdered balanced salt solutions are produced without sodium, and therefore require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation. Please see this protocol for preparing salt solutions from powder concentrates for detailed instructions.PBS vs DPBSPBS and DPBS, both of which include sodium chloride and phosphate buffer, are common reagents used in biological research to maintain pH while minimizing osmotic shock in living cells; however, in contrast to PBS, DPBS also includes potassium chloride and may be formulated with or without calcium and magnesium and with or without glucose and pyruvate. Select PBS or DPBS based on your specific application.
PBS and DPBS selection guide by application
Use this selection guide to find the right PBS and DPBS for your applicationApplicationCa2+, Mg2+Glucose, pyruvatePBS Cat. No.DPBS Cat. No.Cell dissociation, immunostaining––100107001120012700131891201414190 10X: 14200 Powder: 21600Tissue dissection, assay, immunostaining, perfusion++ 14287Immunoprecipitation, immunohistochemistry+– 14040 10X: 14080 Powder: 21300Cell therapy applications–– A1285601A1285602+– A1285801Flow cytometry––A1286301
HBSS vs EBSS
Hanks' balanced salt solution (HBSS) and Earle’s balanced salt solution (EBSS) are both isotonic solutions used to maintain osmolality and pH in biological applications. While both HBSS and EBSS include glucose and sodium bicarbonate for short-term maintenance of cells outside of growth medium, EBSS is designed for use under 5% CO2, whereas HBSS, which is formulated with less sodium bicarbonate, allows for use without CO2. Select from a variety of formulations specific to your application.
View HBSS and EBSS selection guide by application
Use this selection guide to find the right HBSS and EBSS for your applicationApplicationCa2+, Mg2+GlucosePhenol redSodium bicarbonateHBSS Cat. No.EBSS Cat. No.Tissue dissociation, perfusion–+–+1417514155Dissection, staining–+––10X: 14185 Dissection, cell washing–+++14170 Live cell procedure, microscopic study with live cells, dissection++–+14025 Dissection, microscopic study with live cells++––10X: 14065 Cell culture, tissue dissociation++++2402024010+++–10X: 14060
HBSS vs PBS
Hanks’ balanced salt solution (HBSS) and phosphate buffered saline (PBS) are two types of salt solutions commonly used for the maintenance of osmolality and pH in cell culture applications. However, the formulations of these two solution types differ. While PBS is a simple solution containing phosphate buffer, HBSS is more complex and includes sodium bicarbonate. Both may be formulated with glucose and pyruvate as well as calcium and magnesium. However, given their differences, application should be carefully considered when selecting the appropriate solution for your cell culture experiments. See above for selection guides by application.
Browse balanced salt solution products
Phosphate buffered saline (PBS)Commonly used in biological applications, PBS is a water-based salt solution with a simple formulation. It is isotonic and non-toxic for most cells. It includes sodium chloride and phosphate buffer and is formulated to prevent osmotic shock while maintaining living cells’ water balance.
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10010001,10010002,10010023,10010031,10010049,10010072,18912014,20012027,20012043,20012050,70011044,70011069,70013032,70013073,A1286301Dulbecco’s phosphate buffered saline (DPBS)Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.
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14040117,14040133,14040141,14040182,14040216,14080055,14190094,14190136,14190144,14190235,14190250,14190342,14190359,14190367,14200075,14200166,14287072,14287080,21300025,21300058,21300074,21600010,21600044,21600069Hanks' balanced salt solution (HBSS)Originally formulated in 1940 by microbiologist, John Hanks, HBSS is rich in bicarbonate ions. Ordinarily used as buffer systems in cell culture media, HBSS helps maintains optimum physiological pH. Because these buffers are low in reactivity, HBSS is often used in atmospheric conditions. HBSS may be used to maintain osmolality and pH in biological applications.
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14025076,14025092,14025126,14025134,14060040,14065056,14170112,14170120,14170161,14175103, 4175145,14185052,24020117Earle’s balanced salt solution (EBSS)The amount of sodium bicarbonate in EBSS is designed to maintain pH under 5% CO2, while HBSS has lower sodium bicarbonate levels and is intended for incubation without CO2. These buffers also come in a variety of formulations, including with or without calcium/magnesium ions, and with or without phenol red, so you may choose the appropriate buffer depending on your application.
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